| Bacon Creek | | E. coli | 8 | 2011 | Hardin, Hart, Larue | Salt |
| Baughman Fork and UT to Baughman Fork | | Nutrients, Organic Enrichment | 4 | 2000 | Fayette | Kentucky |
| Bayou de Chien, Cooley and Central Creeks | | Fecal Coliform | 3 | 2007 | Graves, Hickman, Carlisle | Mississippi |
| Beargrass Creek | | Fecal Coliform | 6 | 2012 | Jefferson | Salt |
| Beaver Creek | | E. coli | 22 | 2010 | Floyd, Knott | Big Sandy |
| Beech Creek of Pond Creek | | pH | 1 | 2006 | Muhlenberg | Green |
| Brier Creek of Pond River | | pH | 1 | 2000 | Muhlenberg | Green |
| Brush and Crooked Creek - Revision | | E. coli | 1 | 2012 | Rockcastle | Upper Cumberland |
| Brush and Crooked Creeks of Roundstone Creek | | E. coli | 3 | 2006 | Rockcastle | Upper Cumberland |
| Butchers Branch of Blackford Creek | | pH | 1 | 2006 | Hancock | Ohio River |
| Cane Branch of Middle Fork Beaver Creek | | pH | 1 | 2006 | McCreary | Upper Cumberland |
| Cane Creek Watershed | | E. coli, Fecal Coliform | 4 | 2008 | Menifee, Powell | Kentucky |
| Cane Run | | E. coli, Fecal Coliform | 7 | 2013 | Fayette, Scott | Kentucky |
| Cane Run of Caney Creek | | pH | 1 | 2004 | Hopkins | Tradewater |
| Caney, Copper, Hurricane Creeks (Hopkins County) | | pH, Metals | 16 | 2015 | Hopkins | Tradewater |
| Carr Fork | | E. coli | 10 | 2013 | Knott | Kentucky |
| Chenoweth Run | | Nutrients, Aquatic Macrophytes | 2 | 1997 | Jefferson | Salt |
| Clarks River | | E. coli | 40 | 2011 | Calloway, Graves, Marshall, McCracken | Tennessee |
| Copperas Fork of Cooper Creek | | pH | 1 | 2006 | McCreary | Upper Cumberland |
| Cox Creek | | E. coli | 9 | 2013 | Bullitt, Nelson, Spencer | Salt |
| Craborchard Creek of Drakes Creek | | pH | 2 | 2004 | Hopkins | Green |
| Cypress Creek | | pH | 1 | 2010 | Muhlenberg | Green |
| Cypress Creek | | E. coli | 8 | 2011 | Muhlenberg | Green |
| Dix River | | Pathogens | 25 | 2010 | Boyle, Casey, Garrard, Lincoln, Rockcatsle | Kentucky |
| Drakes Creek of Pond River | | pH | 1 | 2006 | Hopkins | Green |
| East Fork Little Sandy | | Organic Enrichment | 1 | 1995 | Lawrence, Boyd, Greenup | Little Sandy |
| Elijahs and Gunpowder Creeks | | Ethylene Glycol | 2 | 1998 | Boone | Ohio |
| Fleming Creek - Revision | | Fecal Coliform | 3 | 2011 | Fleming, Nicholas | Licking |
| Fleming Creek Watershed | | Fecal Coliform | 14 | 2001 | Fleming, Nicholas | Licking |
| Floyds Fork | | Organic Enrichment | 4 | 1997 | Jefferson, Shelby | Salt |
| Floyds Fork | | E. coli, Fecal Coliform | 24 | 2014 | Bullitt, Henry, Jefferson, Oldham, Shelby, Spencer | Salt |
| Hardwick Creek | | E. coli | 5 | 2013 | Powell, Estill | Kentucky |
| Harrods Creek of Ohio River | | Organic Enrichment | 1 | 1995 | Oldham | Ohio |
| Laurel and Little Laurel Rivers | | E. coli | 11 | 2012 | Laurel | Upper Cumberland |
| Little Bayou Creek of Bayou Creek | | PCB in Fish Tissue | 1 | 2001 | McCracken | Ohio |
| Little River Watershed, Lower Cumberland Basin | | Fecal Coliform | 9 | 2009 | Christian, Trigg | Lower Cumberland |
| Lower Cumberland River Basin Stream Segments | | Fecal Coliform | 11 | 2009 | Caldwell, Crittenden, Livingston, Lyon | Lower Cumberland |
| Muddy Creek | | E. coli | 7 | 2013 | Madison | Kentucky |
| Mussin Branch of Moore Creek | | pH | 1 | 2006 | Marion | Salt |
| Newcombe Creek of Little Sandy River | | Total Dissolved Solids, Chlorides | 1 | 2000 | Elliot | Little Sandy |
| North Elkhorn Creek | | E. coli | 3 | 2014 | Fayette | Kentucky |
| North Fork Kentucky River - Cane, Carr and Troublesome Creeks | | E. coli, Fecal Coliform | 22 | 1995 | Breathitt | Kentucky |
| Pleasant Run of Drakes Creek | | pH | 2 | 2004 | Hopkins | Green |
| Pleasant Run of Drakes Creek - Revision | | pH | 1 | 2011 | Hopkins | Green |
| Pond Creek | | E. coli, pH, Metals | 54 | 2017 | Muhlenberg | Green |
| Pond Creek Amendment - Beech Creek | | Copper | 1 | 2017 | Muhlenberg | Green |
| Pond Creek of Green River | | pH | 4 | 2007 | Muhlenberg | Green |
| Render Creek of Lewis Creek | | pH | 1 | 2006 | Ohio | Green |
| Rock Creek of S Fork Cumberland, White Oak Creek | | pH | 2 | 2006 | McCreary | Upper Cumberland |
| Ryans Creek of Jellico Creek | | pH | 1 | 2006 | McCreary, Whitley | Upper Cumberland |
| South Elkhorn Creek | | E. coli, Fecal Coliform | 11 | 2013 | Fayette, Franklin, Jessamine, Scott, Woodford | Kentucky |
| South Fork Red River - Revision | | Chlorides | 1 | 2011 | Powell | Kentucky |
| South Fork Red River, Sand Lick Fork, Stump Cave Branch | | Total Dissolved Solids | 4 | 1999 | Powell | Kentucky |
| Sugar Creek Watershed of Clear Creek | | pH | 1 | 2004 | Hopkins | Tradewater |
| Taylorsville Lake | | Nutrients | 1 | 2000 | Spencer | Salt |
| Townsend Creek | | Bacteria | 4 | 2011 | Bourbon, Harrison | Licking |
| Unnamed Tributary to Rolling Fork | | pH | 1 | 2006 | Marion | Salt |
| Unnamed Tributary to South Fork Russell Creek | | Total Dissolved Solids | 1 | 2000 | Green | Green |
| Upper Cumberland River and Tributaries | | Fecal Coliform | 26 | 1998 | Harlan, McCreary, Bell, Knox, Pulaski, Whitley | Upper Cumberland |
| Upper Green River | | Fecal Coliform | 15 | 2008 | Adair, Butler, Edmonson, Graves, Grayson, Green, Hardin, Hart, Taylor, Warren | Green |
| Upper Green River II | | Fecal Coliform | 20 | 2009 | Adair, Green, Hart, Metcalfe, Taylor | Green |
| Wildcat Branch of Cumberland River | | pH | 1 | 2006 | Pulaski | Upper Cumberland |